Isolation of the aldobionic acid of umbilical cord hyaluronic acid.

In a preliminary note there was outlined the isolation of a crystalline glucuronido-glucosamine disaccharide, designated hyalobiuronic acid, from umbilical cord hyaluronate by combined enzymatic and partial mineral acid hydrolysis (1). In subsequent articles the use of this compound as a basis for structural work on the polysaccharide and as a reference point for interpretation of enzymatic dat.a will be described. Here, it is desired to amplify the earlier account of the isolation and characterization of the disaccharide, to offer evidence of its homogeneity, and to describe briefly its production by other methods.